Expert User Database

Want to learn a new technique or take advantage of equipment with training provided?

Simply contact the expert user identified in this table and arrange a time to receive this training.

Database last updated: March 2017.

Technique Procedure Quantitative Equipment Primary Contact Lab Email Secondary Contact Lab Email
Apoptosis Annexin V Yes Beckman CytoFlex Flow Cytometer Mark Boyd Lab Manager mark.boyd@usask.ca Shari Smith Anderson ses747@mail.usask.ca
  TUNEL   Fluorescent Microscope (Nikon Eclipse TE300 or E400, Olympus 1X51)            
Bacterial Protein Expression Cloning No   Raghu Goel Lukong rkg062@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Culture No Thermo MaxQ Shaking Incubator Jeremy Marshall Anderson jem826@mail.usask.ca Raghu Goel Lukong rkg062@mail.usask.ca
  Mutagenesis No   Raghu Goel Lukong rkg062@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Optical Density Yes Pharmacia Biotech Ultraspec 3000 Spectrophotometer Jeremy Marshall Anderson jem826@mail.usask.ca Raghu Goel Lukong rkg062@mail.usask.ca
  Protein Extraction No Ultracentrifuge (Beckman J2-21M, J2-MI, Optima L-100 XP) Jeremy Marshall Anderson jem826@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
Cell-based Assays Adhesion Yes   Paul Mellor Anderson paul.mellor@usask.ca Shari Smith Anderson ses747@mail.usask.ca
  Chemotaxis/Migration Yes   Paul Mellor Anderson paul.mellor@usask.ca Shari Smith Anderson ses747@mail.usask.ca
  Foci Formation Yes   Paul Mellor Anderson paul.mellor@usask.ca Shari Smith Anderson ses747@mail.usask.ca
  Hypoxia Yes Hypoxic Chamber Paul Mellor Anderson paul.mellor@usask.ca Shari Smith Anderson ses747@mail.usask.ca
  In-cell Western Yes LICOR Odyssey Infrared Scanner          
  Invasion/Wound Healing Yes   Paul Mellor Anderson paul.mellor@usask.ca      
  MTT Assay Yes Molecular Devices Spectra Max M5 Plate Reader Paul Mellor Anderson paul.mellor@usask.ca      
  Non-adherant Growth (soft agar assay) Yes   Paul Mellor Anderson paul.mellor@usask.ca Shari Smith Anderson ses747@mail.usask.ca
Cell cycle assay Cell cycle assay, fluorescence-based Yes Beckman CytoFlex Flow Cytometer Mark Boyd Lab Manager mark.boyd@usask.ca      
Confocal Microscopy Fixed-cell Imaging No Zeiss LSM 700 Confocal Microscope Shari Smith Anderson ses747@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Live Cell Imaging No Zeiss LSM 700 Confocal Microscope Shari Smith Anderson ses747@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Co-localization Yes Zeiss LSM 700 Confocal Microscope Shari Smith Anderson ses747@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Z-stacks No Zeiss LSM 700 Confocal Microscope Sreejit Parameswaran Vizeacoumar sreejit.p@usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Time-lapse Imaging No Zeiss LSM 700 Confocal Microscope Shari Smith Anderson ses747@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
DNA Quantification Flow Cytometry (intact cells) Yes Beckman CytoFlex Flow Cytometer Mark Boyd Lab Manager mark.boyd@usask.ca Shari Smith Anderson ses747@mail.usask.ca
  NanoDrop (extracted DNA) Yes Nanodrop 2000C Paul Mellor Anderson paul.mellor@usask.ca Stephanie Kendall Anderson scj503@mail.usask.ca
DNA Sequencing PCR Product Template No Applied Biosystems 2720 or Veriti Thermocycler Paul Mellor Anderson paul.mellor@usask.ca Stephanie Kendall Anderson scj503@mail.usask.ca
  Plasmid Template No   Paul Mellor Anderson paul.mellor@usask.ca Raghu Goel Lukong rkg062@mail.usask.ca
Methylation Sequencing (bisulfite treatment) No Applied Biosystems 2720 or Veriti Thermocycler Stephanie Kendall Anderson scj503@mail.usask.ca Shari Smith Anderson ses747@mail.usask.ca
  Sequence Analysis No Mac Vector software Shari Smith Anderson ses747@mail.usask.ca Mark Boyd Lab Manager mark.boyd@usask.ca
Drying Speed Vacuum No Labconco Centrivap Speed Vacuum Mark Boyd Lab Manager mark.boyd@usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
Enzyme Assays kinetic assays Yes GloMax® 96-well microplate luminometer w/ dual injectors (Promega) Mark Boyd Lab Manager mark.boyd@usask.ca      
  dual gene reporter assays Yes GloMax® 96-well microplate luminometer w/ dual injectors (Promega) Mark Boyd Lab Manager mark.boyd@usask.ca      
  luciferase assays Yes GloMax® 96-well microplate luminometer w/ dual injectors (Promega) Mark Boyd Lab Manager mark.boyd@usask.ca      
  Cell viability/ATP assays Yes GloMax® 96-well microplate luminometer w/ dual injectors (Promega) Mark Boyd Lab Manager mark.boyd@usask.ca      
  Intracellular Ca+2 assays Yes GloMax® 96-well microplate luminometer w/ dual injectors (Promega) Mark Boyd Lab Manager mark.boyd@usask.ca      
  Immunoassays Yes GloMax® 96-well microplate luminometer w/ dual injectors (Promega) Mark Boyd Lab Manager mark.boyd@usask.ca      
Flow cytometry Immunodetection of up to 9 colors/targets Yes Beckman CytoFlex Flow Cytometer Mark Boyd Lab Manager mark.boyd@usask.ca      
Fluorescence Fluorescent Gel Imaging (protein and DNA) Yes BioRad ChemiDoc MP Imaging System Mark Boyd Lab Manager mark.boyd@usask.ca Robin Love Chelico music.robin@gmail.com
  Fluorescent Western Blot Imaging Yes BioRad ChemiDoc MP Imaging System Mark Boyd Lab Manager mark.boyd@usask.ca Robin Love Chelico music.robin@gmail.com
    Yes Molecular Devices Spectra Max M5 Plate Reader Tanya Freywald Freywald (4D10) tanya.freywald@usask.ca Paul Mellor Anderson paul.mellor@usask.ca
Gene Expression Analysis Microarray analysis Yes Affymetrix GeneChip GCS3000 Frederick Vizeacoumar Vizeacoumar frederick.vizeacoumar@usask.ca      
Gene Knockdown shRNA libraries, individual genes or pooled   shRNAs, each gene in human genome, 3-5 shRNA/gene; & pooled; lentiviral Frederick Vizeacoumar Vizeacoumar frederick.vizeacoumar@usask.ca      
Gene Knockout CRISPR library, individual genes, whole genome   CRISPR, each gene in human genome; lentiviral Frederick Vizeacoumar Vizeacoumar frederick.vizeacoumar@usask.ca      
High-throughput imaging Fluorescence imaging in 96-well format No ImageXpress Micro XLS Widefield and Ultra Confocal High-Content Microscopes Frederick Vizeacoumar Vizeacoumar frederick.vizeacoumar@usask.ca      
Immunohisto Chemistry Slide Preparation No   Shari Smith Anderson ses747@mail.usask.ca Stephanie Kendall Anderson scj503@mail.usask.ca
  Probing No   Shari Smith Anderson ses747@mail.usask.ca Stephanie Kendall Anderson scj503@mail.usask.ca
  Imaging Semi Fluorescent Microscope (Nikon Eclipse TE300 or E400, Olympus 1X51) Shari Smith Anderson ses747@mail.usask.ca      
  Imaging Yes Zeiss LSM 700 Confocal Microscope Sreejit Parameswaran Vizeacoumar sreejit.p@usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
Microarray deconvolution of synthetic lethal screen data Yes Affymetrix GeneChip GCS3000 Chelsea Cunningham Vizeacoumar cec120@mail.usask.ca Frederick Vizeacoumar Vizeacoumar frederick.vizeacoumar@usask.ca
PCR Primer Design No Primer3 and MacVector Software Paul Mellor Anderson paul.mellor@usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  PCR Optimization No Applied Biosystems 2720 Thermocycler Paul Mellor Anderson paul.mellor@usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  PCR Optimization (gradient) No Applied Biosystems Veriti gradient thermocycler Paul Mellor Anderson paul.mellor@usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Agarose Gel Elecrophoresis No Gel Beds, VWR Accu Power 300 Power Supply Stephanie Kendall Anderson scj503@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Gel Imaging No BioRad Gel Doc, Quanti One software Stephanie Kendall Anderson scj503@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
PCR, Digital Droplet Copy Number Variation Yes BioRad QX100 Droplet Generator and Droplet Reader (Lab 2D10) Brooke Thompson Kirychuk (2D10) bnt576@mail.usask.ca      
Mutation Detection Yes BioRad QX100 Droplet Generator and Droplet Reader (Lab 2D10) Brooke Thompson Kirychuk (2D10) bnt576@mail.usask.ca      
  Quantitation Yes BioRad QX100 Droplet Generator and Droplet Reader (Lab 2D10) Brooke Thompson Kirychuk (2D10) bnt576@mail.usask.ca      
PCR, Real-time  RNA Extraction No   Stephanie Kendall Anderson scj503@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Reverse Transcription No Applied Biosystems 2720 or Veriti Thermocycler Stephanie Kendall Anderson scj503@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Real-time PCR Yes Applied Biosystems StepOne Plus instrument Stephanie Kendall Anderson scj503@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Data Analysis Yes Applied Biosystems StepOne Plus software Stephanie Kendall Anderson scj503@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
Protein Purification Purification No Akta Purifier Jeremy Marshall Anderson jem826@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
  Quantification Yes NanoDrop 2000C Jeremy Marshall Anderson jem826@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
Protein Quantificaiton Bradford (lysate) Yes Pharmacia Biotech Ultraspec 3100 Pro Spectrophotometer Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Bradford (lysate) Yes Molecular Devices Spectra Max M5 Plate Reader Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  ELISA Yes Molecular Devices Spectra Max M5 Plate Reader Tanya Freywald Freywald (4D10) tanya.freywald@usask.ca      
  Flow Cytometry (intact cells) Yes Coulter EPICS XL Flow Cytometer Mark Boyd Lab Manager mark.boyd@usask.ca Shari Smith Anderson ses747@mail.usask.ca
Lowry (lysate) Yes Pharmacia Biotech Ultraspec 3100 Pro Spectrophotometer Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Lowry (lystate) Yes Molecular Devices Spectra Max M5 Plate Reader Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  NanoDrop (lysate) Yes Nanodrop 2000C Jeremy Marshall Anderson jem826@mail.usask.ca Dielle Whitecross Anderson ded442@mail.usask.ca
Radio Isotope Assays ATPase Assay Yes Beckman LS 6000IC Scintillation Counter Hao Ding Wu dih078@mail.usask.ca      
EMSA Yes Beckman LS 6000IC Scintillation Counter Hao Ding Wu dih078@mail.usask.ca      
  Helicase Assay Yes Beckman LS 6000IC Scintillation Counter Manhong Guo Wu mag919@mail.usask.ca      
  GAP Assay Semi   Dielle Whitecross Anderson ded442@mail.usask.ca      
  Kinase Assay Semi   Dielle Whitecross Anderson ded442@mail.usask.ca      
  Surface Wipe Test Yes Beckman LS 6000IC Scintillation Counter Dielle Whitecross Anderson ded442@mail.usask.ca      
Reporter Assays Luminometer Yes GloMax® 96-well microplate luminometer w/ dual injectors (Promega) Mark Boyd Lab Manager mark.boyd@usask.ca      
Western Blot Protein Extraction (whole cell lysate) >No   Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  SDS-PAGE No BioRad gel tanks, power packs Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Transfer (wet) No BioRad Mini Protean II Raghu Goel Lukong rkg062@mail.usask.ca      
  Transfer (semi-dry) No Fisher Semi-Dry Blotting Unit FB-SDB-2020 Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Transfer (semi-dry) No BioRad Trans-Blot Turbo Transfer System Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Probing No   Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Detection (ChemiDoc) Semi BioRad Chemi Doc XRS, Quanti One software Dielle Whitecross Anderson ded442@mail.usask.ca      
  Detection (chemiluminescence) Semi BioRad ChemiDoc MP Imaging System Raghu Goel Lukong rkg062@mail.usask.ca      
  Detection (infrared scanner) Yes LICOR Odyssey Infrared Scanner Dielle Whitecross Anderson ded442@mail.usask.ca Paul Mellor Anderson paul.mellor@usask.ca
  Detection (phospho imager) Semi Typhoon FLA Phosphorimager Dielle Whitecross Anderson ded442@mail.usask.ca Manhong Guo Wu mag919@mail.usask.ca