Shared Equipment

To arrange access to the Cancer Cluster instruments please contact:

Mark Boyd
Lab Manager of 4th floor, and 6th Floor
Office Room 4B30
Phone: (306) 370-1296
E-mail: mark.boyd@usask.ca

See Expert User Database for contact information for an expert user that can provide the necessary training needed to use the equipment for the particular application needed.

If the data obtained using the Cancer Cluster instrumentation are used for publication, please include the statement below in the acknowledgements section:
"Research described in this paper was performed in part at the Cancer Research Cluster, which is supported by the Saskatchewan Cancer Agency and the College of Medicine, University of Saskatchewan."

ChemiDoc
(Molecular Imager ChemiDoc XRS System)
  • UV detection for DNA gels
  • white light imaging
  • chemilluminescence digital capture and quantification for Westerns/Immunoblots
Confocal Microscope
(Zeiss LSM700 system)
  • User fees apply, $30/hr
  • immunofluorescence microscopy focused on one 'layer' of the cell
  • can build 3-D images by taking multiple Z-stacks
  • coimmunofluorescence and colocalization using software analysis
  • fixed or live cells imaging
  • video imaging capability to capture imaging over time
  • multiple fluores can be used in the same sample and the same time
  • GFP. RFP, CFP. YFP, DAPI, brightfield
  • Alexa dyes, Cy dyes
  • spectral unmixing capabilities, even for dyes with overlapping spectra
  • FRET, FRAP experiments
  • significant training required

Cytoflex Flow Cytometer
(Beckman CytoFLEX Flow Cytometer)
  • User fees apply, $50/hr
  • 3 lasers; 405 nm, 638 nm, 405 nm
  • capable of detecting 9 colors at once
  • 96-plates or single samples
  • Quantification of cell surface markers or intracellular proteins
  • Requires antibodies to target protein
  • max three colours at one time
  • Cell cycle analysis
  • Apoptosis assays

Fluorescence Microscope System (permission of owner Dr. Lukong required)
(Olympus IX51)

  • immunofluorescence microscopy
  • green, red, blue, brightfield
  • e.g. GFP, RFP, DAPI, light images
  • photography capability
Biorad Imager

 

Biorad Imaging System
(ChemiDoc MP Imagaing System - 17001402)

  • multiplex fluorescent western blotting
  • chemiluminescence detection
  • general gel documentation applications and stain-free technology
  • fluorescent reporters (RGB, far red, and near infrared) including StarBright™ Fluorophores
  • detect up to three proteins simultaneously
  • features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images
  • stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
  • three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • White Sample Tray, for gels stained with Coomassie Blue, copper, silver, or zinc stains
  • Trans-UV, 302 nm
    Epi-white
    Trans-white (White Sample Tray required)
    Trans-blue, 450-490 nm
    Epi-blue, 460-490 nm excitation
    Epi-green, 520-545 nm excitation
    Epi-red, 625-650 nm excitation
    Epi-far red, 650-675 nm excitation; so suitable for IR-680 dyes (like LICOR)
    Epi-near IR, 755-777 nm excitation; so suitable for IR-800 dyes (like LICOR)
LICOR Odyssey Scanner
  • quantitative Western/Immunoblots for protein detection
  • much less expensive than chemiluminescence (no film, no chemi solutions)
  • uses infrared-linked secondary antibodies (very economical)
  • directly scans blots to provide accurate quantification over several orders of magnitude
  • more sensitive detection than film (from chemi)
  • digital image of same quality as film (from chemi)
  • two channels to detect two species of antibodies at the same time (e.g. mouse and rabbit)
  • in-cell Western to carry out higher throughput analyses in 96-well format for validated antibodies that only recognize one protein in cells
Luminometer

GloMax(R) 96 Microplate Luminometer w/ Dual Injectors from Promega
  • chemiluminescent and bioluminescent applications
  • highly sensitive and quantitative; nine logs of dynamic range
  • capable of performing both flash and glow-type luminescent assays
  • injector volume range from 25 - 250 ul in 1-ul increments
  • software wizards assist with protocol development
  • Suitable for: dual reporter assays, steady-Glo® Luciferase Assays, Flash Glow Luciferase Assays, Cell Viability/ATP Assays, Kinetic Assays, intracellular Ca+2 Assays, Immunoassays
  • Spectral Range: 350-650 nm
  • Detection Limit: 3 x 10-21 moles of luciferase
  • Requires the use of 96-well microplates
  • Data exports into Excel spreadsheet
MacVector software
(PowerMac and software workstation)
  • for analysis of DNA and/or protein sequences
  • sequence alignments (DNA or protein)
  • restriction analysis predictions
  • protein properties (pI, molecular weight, hydrophobicity)
Microarray system (permission of Dr. Vizeacoumar required)
(Affymetrix GeneChip GCS3000)
  • Possible User fees
  • Users must purchase the appropriate compatible chips and reagents (see Dr. Vizeacoumar)
  • analysis of gene expression
  • synthetic lethal shRNA library deconvolution
  • significant training required or may need to arrange expert user to run/help with sample analyses
Nanodrop
(Nanodrop 2000C)
  • determination of protein or nucleic acid concentrations
  • uses very small sample sizes

Phenogenomic Imaging Centre of Saskatchewan
-gene knockdown (shRNA) and gene knockout (CRISPR) technology

-high-content screening and imaging capabilities

  • User fees apply
  • ImageXpress Micro XLS Widefield High Content Screening System
  • ImageXpress Ultra Confocal High-Content Analysis System
  • Capable of fluorescence imaging of 96-well plates
  • Pooled shRNA library, lentiviral delivery
  • Arrayed shRNA library, lentiviral delivery
  • CRISPR-Cas9 gene editing tehcnology
  • See above link for more information

Typhoon Phosphorimager
(Typhoon FLA 7000, from GE Healthcare)
  • Typhoon FLA7000 Scanner Main Bi-Alkali PMT Laser
    LD 635 Laser LD 650 Laser LD 473 Laser SHG 532 Y520 filter (FLA7k) O580 filter (FLA7k) R670
    filter (FLA7k) IP filter (FLA7k) Fluor stage (FLA7k) Membrane weight (FLA7k) Phosphor stage
    (FLA7k) Fluorescent plate Capture software
  • digital detection and quantification of 32P and 35S-based radioactive assays on gels and/or TLC plates; requires high resolution phosphor screens to capture information. Some shared and available for use from Deborah Anderson (deborah.anderson@saskcancer.ca)
  • Fluorescent gel imaging of DNA and Protein
  • Fluorescent Western blot imaging
Real-Time PCR
(Applied Biosystems StepOne Plus)
  • quantitative mRNA expression level measurements
  • Users must purchase the appropriate reagents that are compatible with this machine (see Mark Boyd)
Scintillation Counter
(Beckman LK 6000IC Scintillation Counter)
  • liquid radio-isotope measurements
  • 32P, 125I, 35S, 3H, 14C
  • experimental measurements
  • wipe test results
SpectroMax M5 Fluorescence Microplate Reader (and cuvette too)
  • spectrophotometer for single cuvette or 96-well plate formats
  • absorbance and fluorescence capabilities
  • tuneable wavelengths for excitation and emission
  • suitable for many types of assays and fluorescence measurements
Speed Vac
  • to dry samples under vacuum in 1.5 mL Eppendorf sized tubes
Ultracentrifuge
(Optima L-100 XP Ultracentrifuge)
  • very high speed centrifugation, under vacuum